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KMID : 0370220030470040230
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Yakhak Hoeji
2003 Volume.47 No. 4 p.230 ~ p.233
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Effects of Protoberberine Alkaloids on L-DOPA-Induced Cytotoxicity in PC12 Cells
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이재준/Lee JJ
김유미/김춘매/양유정/강민희/이명구/Kim YM/Jin CM/Yang YJ/Kang MH/Lee MK
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Abstract
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Previously, Protoberberine alkaloids such as berberine and palmatine have been found to lower dopamine content in PCl2 cells (Shin et al., 2000). In this study, the eff ects of berberine and palmatine on L-DOPA-induced increase in dopamine level and cytotoxicity in PCl2 cells were investigated. Treatment of PCl2 with L-DOPA at concentration ranges of 20~50 μM increased dopamine content and the increase in dopamine levels by L-DOPA was inhibited by 10-40 μM berberine and 10~80 μM palmatine, which the concentration ranges did not show a cytotoxicit7. However, berberine and palmatine at concentrations higher than 50 μM and 100 μM caused a cytotoxicity respectively, In addition, berberine (10~20 μM) and palmatine (10~50 μ M) at non-cytotoxic concentration ranges ageavated L-DOPA-induced cytotoxicity in PCl2 cells (L-DOPA concentration ranges,20~50 μM). The L-DOPA-induced cytotoxicity was also siglificantly potentiated by berberine (50 μM) and palmatine (100 μM) with cytotoxic ranges. These data demonstrate that berberine and palmatine inhibit L-DOPA- induced increase in dopamine content and stimulate L-DOPA-induced neurotoxicity Therefore, the portsibility that the long-term L-DOPA treated patients with berberine and palmatine could be checked the adverse symptoms.
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KEYWORD
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